Journal: Vaccines

Article Title: Re-Evaluation and Retrospective Comparison of Serum Neutralization Induced by Three Different Types of Inactivated SARS-CoV-2 Vaccines

doi: 10.3390/vaccines12111204

Figure Lengend Snippet: Serum neutralization against several newly emerged Omicron variants. ( A ) Pseudovirus-dependent in vitro neutralization assays against wildtype SARS-CoV-2, Delta, Omicron (B.1.1.529), BA.2.75, BA.4, BF.7, BQ.1.1, XBB pseudoviruses in the presence of different serum dilutions of the selected top 11 neutralizers. The highest serum concentration ( x -axis) was 2.5% with a 1:2 serial dilution for eight dilutions in total. The neutralization data were analyzed based on the relative luciferase activity ( y -axis) using Prism software by nonlinear regression (curve fit) method. ( B ) A heat map for serum NT 50 values of the selected top 11 neutralizers. Each row represents a serum sample, while each column represents neutralization assays against an indicated SARS-CoV-2 variant. Based on the performed pseudovirus-dependent in vitro neutralization assays in ( A ), the serum neutralization titer NT 50 value for each serum sample against a certain SARS-CoV-2 variant was calculated using Prism software. Higher NT 50 value means more potent serum neutralizing activity. The serum exhibiting no neutralization activity has NT 50 lower than 100. ( C ) Comparison of the calculated NT 50 values between wildtype SARS-CoV-2 and the other different variants. The p -values were calculated using the Friedman test and Dunn’s multiple comparisons test, and no significant difference (n.s.) among the NT 50 values for wildtype SARS-CoV-2, and Delta and Omicron variants was observed.

Article Snippet: In order to assess the statistical significance for comparisons, the Mann–Whitney test or Kruskal–Wallis test was performed by using PRISM software (GraphPad 9.5.0).

Techniques: Neutralization, In Vitro, Concentration Assay, Serial Dilution, Luciferase, Activity Assay, Software, Variant Assay, Comparison